Fig. 4
From: The homeodomain transcription factor Orthopedia is involved in development of the Drosophila hindgut
Generation of the otp GT allele and molecular analysis of the otp1024 allele. a At the top, the genomic organization of the otp gene is shown with exons indicated as gray boxes. The otp gene targeting construct in the targeting vector pW25 (otpGT-pW25) is shown below (FRT, FLP recombination target sequences; I-site, I-SceI recognition site; Whs, white gene with hsp70 promoter). Further down the otp genomic region after the gene targeting event is indicated (otpGT); here part of exon 4 (containing the translation start) and the complete exon 5 (containing most part of the homeobox) are replaced by the white gene of the targeting construct. b Nucleotide and amino acid sequences in wild-type (here otp-RC as an example) and mutant DNA. EMS induced a G to A transition in the coding region of otp1024 leading to the formation of a stop codon. c Schematic overview of the wild-type Otp protein with the localisation of the homeodomain (yellow) in comparison to the truncated mutant protein of the otp1024 allele