Fig. 1
From: The homeodomain transcription factor Orthopedia is involved in development of the Drosophila hindgut
Hindgut and nervous system specific transcripts of the orthopedia (otp) gene. a Genomic organisation of the otp gene with exons indicated as gray boxes and the regulatory region for the otp hindgut expression with the Byn binding sites (hindgut module) [31]. Below the two different transcripts (otp-RE and otp-RC) are shown (gray, untranslated region; blue, translated region; yellow, homeobox). The specific genomic probes (otp 1 and otp 2) used to detect otp-RE and otp-RC by in situ hybridisation are indicated. b-j Embryonic expression pattern of the different otp transcripts. The stages shown (9, 10, and 14) were determined according to Campos-Ortega and Hartenstein (1997) [1], all views are lateral views, anterior is to the left. b-d Whole-mount in situ hybridisation performed with an otp cDNA probe against exons 3–9 detecting all transcript forms (otp-RC). The boundary between stronger anterior expression and weaker posterior expression in the nervous system is indicated by a blue arrowhead, the weaker expression in the rectum compared with the rest of the hindgut by a black arrowhead in d. e-g In situ hybridisation performed with a probe against exon 1 and 2 of the otp gene (otp 1). h-j In situ hybridisation performed with a probe against exon 3 of the otp gene (otp 2)