Table 2 Characteristics of the 29 EST microsatellite markers and population genetic diversity analysis for Shuangyang Sika deer
M | Repeat Motif | Primer Sequences (5′–3′) | Tm | Size (bp) | Putative Function | Na | Ne | He | Ho | PIC |
|---|---|---|---|---|---|---|---|---|---|---|
M001 | (TC)10 | F: M13-TTCCCTTCTGGTTCTCAATCTC | 60 | 246–252 | Nop14-like family | 4 | 3.1 | 0.6751 | 0.6496 | 0.6142 |
R: CTGACTCTCACGTCTAAGCTCTTCT | ||||||||||
M002 | (AC)10 | F: M13-TATGAAAGGGCCTGGTTGTG | 60 | 346–354 | No significant match | 4 | 1.1 | 0.3772 | 0.2791 | 0.2758 |
R: CCACCATGTAGCCAGAATGG | ||||||||||
M005 | (CT)10 | F: M13-AATCGTATCACAGCACGGCT | 60 | 282–288 | No significant match | 3 | 2 | 0.4983 | 0.518 | 0.4414 |
R: CCCTGGTTAAGAGTCCCAGAT | ||||||||||
M009 | (GA)7cag(GA)10 | F: M13-CCACTAACTCTCCTGGCTGC | 60 | 357–363 | No significant match | 4 | 2.4 | 0.5807 | 0.5333 | 0.5178 |
R: AAGATGGTTTACTCAGTAATCGATG | ||||||||||
M027 | (AT)8ttgaatccctctcctaaagcacagtatcttatataatactgtgattgcattga(TC)9 | F: M13-TTCCTCACTTTCCTCATACATCTG | 60 | 251–321 | No significant match | 12 | 2.1 | 0.5215 | 0.375 | 0.5029 |
R: ATCTTCCAGTCAGGTGTTCAGC | ||||||||||
M037 | (AC)9ccc(CA)8 | F: M13-TCCCTCTAGCTGGAGCAATC | 60 | 165–187 | Bos taurus cytochrome P450 2C21-like | 9 | 4.6 | 0.7845 | 0.6547 | 0.7507 |
R: CTCTTGACAGTTTGGCATGTATAGT | ||||||||||
M073 | (AT)10 | F: M13-TTCAAATGAAGAAAGCTGAATG | 60 | 109–113 | Capra hircus family with sequence similarity 210 | 8 | 2.9 | 0.6546 | 0.5839 | 0.6031 |
R: GTAAAAGTCACTTTTAAAGGGTCA | ||||||||||
M079 | (CT)10 | F: M13-CCTTAAAAGCATCATTTGAGCA | 60 | 157–169 | Gorilla gorilla gorilla transcription factor AP-2 alpha | 5 | 2.8 | 0.649 | 0.6423 | 0.6083 |
R: GCTATAACTGCTTCCAAAACGG | ||||||||||
M082 | (AT)10 | F: M13-CCATGTTTCTATTGGGTTGTCG | 60 | 374–408 | No significant match | 14 | 3 | 0.6708 | 0.5547 | 0.6462 |
R: AACTGGATCTACACTTCACACGGT | ||||||||||
M084 | (GT)10 | F: M13-AGGTCTTCCCCAGTGATGATG | 60 | 422–424 | No significant match | 2 | 1.4 | 0.3087 | 0.292 | 0.2602 |
R: GAGTCAATATCTGGAGCTCTGCC | ||||||||||
M089 | (GA)10 | F: M13-TTCCCTTCTGGTTCTCAATCTC | 60 | 223–229 | Pantholops hodgsonii thyroglobulin gene | 4 | 3 | 0.6705 | 0.6475 | 0.6093 |
R: CTCCTGTGTTGCTCACTCTCATC | ||||||||||
M092 | (AC)10 | F: M13-CCACCATCGGCGAAGAGTC | 60 | 219–235 | No significant match | 5 | 2.7 | 0.6253 | 0.6331 | 0.5638 |
R: TGCTGAGCCGACTTCCTGC | ||||||||||
M093 | (TG)10 | F: M13-TGTTGCTTGGGCTGTGGC | 60 | 149–161 | No significant match | 6 | 2.1 | 0.532 | 0.4892 | 0.4623 |
R: GTGGGCCTTAATCCAGCAAG | ||||||||||
M099 | (AT)10 | F: M13-ACAAATGGTGAAAACTCACTCC | 60 | 202–208 | Bos taurus probable E3 ubiquitin-protein ligase MID2-like | 3 | 2.7 | 0.6281 | 0 | 0.5475 |
R: GGTTCCTTAGTATCCTTGGCAC | ||||||||||
M102 | (AT)10 | F: M13-GGCTGCCTCGCTCTGTTCT | 60 | 249–259 | No significant match | 6 | 3.3 | 0.7033 | 0.6763 | 0.6518 |
R: GCGATGGATGAACATTCAAGAA | ||||||||||
M110 | (GT)10 | F: M13-GTTGGCTCTTGGTCCCTGAAT | 60 | 155–177 | Homo sapiens chromosome 5 clone CTD-2410 N18 | 9 | 3.4 | 0.7127 | 0.6449 | 0.665 |
R: ATCCTCCTCCAGATTCTCCACTT | ||||||||||
M113 | (GA)10 | F: M13-AGGTTCCATCCTTGCCCTG | 60 | 313–329 | No significant match | 6 | 2.9 | 0.6549 | 0.6544 | 0.605 |
R: AACTTAGCCCAGTGAAATGCG | ||||||||||
M114 | (AT)10 | F: M13-GCTGGGTGACAAAGTGCTATG | 60 | 166–176 | Capra hircus family with sequence similarity 210 | 5 | 2.8 | 0.6477 | 0.6187 | 0.5962 |
R: TGAAGAAAGCTGAATGAAAGTTG | ||||||||||
M117 | (AC)10 | F: M13-CACTGCATGCCTGCTTCCT | 60 | 193–207 | Pantholops hodgsonii snail family zinc finger 2 | 4 | 2.1 | – | – | – |
R: TTTACTTGTCACTCCGCCTCTG | ||||||||||
M121 | (CT)10 | F: M13-GATCTCCCAGCCTGTCCTATG | 60 | 251–255 | No significant match | 3 | 1.5 | 0.3413 | 0.3165 | 0.2912 |
R: ACACTTCCCTGAGCTGACCTTAT | ||||||||||
M131 | (AG)10 | F: M13-CGAGTTCTGCTCATTGATGTG | 60 | 164–172 | Ovis aries Rho GTPase activating protein 19 | 3 | 2.3 | 0.5758 | 0.5827 | 0.4829 |
R: TCCTGTGACTCAACTGATACCTC | ||||||||||
M132 | (CT)10 | F: M13-GAGTTCTGCTCATTGATGTGCT | 60 | 149–157 | Ovis aries Rho GTPase activating protein 19 | 3 | 2.3 | 0.5758 | 0.5827 | 0.4829 |
R: GATACCTCTAACCTCACTCAGCG | ||||||||||
M136 | (CA)10 | F: M13-GACTCTTGGTCACAGCAGTCAC | 60 | 207–221 | Bos taurus SET domain containing 1B | 5 | 2.3 | – | – | – |
R: ACTTCTGTACATAGGCTGCCAT | ||||||||||
M139 | (AG)10 | F: M13-GCAGGAGTGAAAGGCAGATG | 60 | 177–187 | Bos taurus synapse differentiation inducing 1-like | 6 | 2.5 | 0.6036 | 0.6475 | 0.5435 |
R: GGATTCTGCACTCAGTGGCT | ||||||||||
M148 | (CT)10 | F: M13-TGATGCGTTCCTCTGTCAGC | 60 | 383–385 | Capra hircus transmembrane protein 164 | 4 | 2.1 | 0.5198 | 0 | 0.4682 |
R: AATGGTCTTCCACTAGCCGC | ||||||||||
M157 | (TC)10 | F: M13-TTCTGACTGAGGAAGCGTCC | 60 | 184–202 | Homo sapiens chromosome 17, clone RP11-960B9 | 6 | 4.2 | 0.7644 | 0.7698 | 0.7265 |
R: GGTCCCATGACTTAGCCTTAC | ||||||||||
M159 | (CA)10 | F: M13-TTCCAAACGCCAGAGGTAAC | 60 | 207–215 | Bos mutus ankyrin repeat domain 13A | 5 | 3 | 0.6692 | 0.6763 | 0.6059 |
R: GCTAGTTGAAGGATATGCAGGC | ||||||||||
M174 | (AG)10 | F: M13-CTTGTTGGAGGATGGATGGTT | 60 | 120–132 | Bos taurus protein inhibitor of activated STAT | 4 | 2.3 | 0.5636 | 0.5683 | 0.4974 |
R: TCCAAGGACAAGTGTTAGAAAGG | ||||||||||
M176 | (AG)10 | F: M13-GACACCACTTCTTGCCTCAAT | 60 | 279–297 | Bos taurus filamin B, beta | 10 | 3.8 | 0.7391 | 0.252 | 0.6947 |
R: ATTAAGCCACTTGTCTCTACAGG | ||||||||||
Average | – | – |  | – | – | 5.6± 2.8 | 2.6± 0.8 | 0.6018 ± 0.1190 | 0.5127 ± 0.2010 | 0.5450 ± 0.1262 |