Fig. 2

The identification of transgenic tobacco lines and their phenotype under PEG-simulated drought stress. a The electrophoresis of the product after PCR amplification of the Ht1-FFT gene in transgenic and WT tobacco lines. Lanes: M: DNA marker; WT: wild type; TL1 to TL3: transgenic lines; P: positive control (plasmid). b The mean ± SE relative transcript level of Ht1-FFT in the leaves of transgenic lines, using actin gene as the internal control. WT: wild type; TL1 to TL3: transgenic lines; any two lines with a different lowercase letter are significantly different (P < 0.05), using Tukey’s honestly significant difference. c Tobacco seedlings under PEG-simulated drought stress treatment (20% PEG). WT: wild type; TL1 to TL3: putative transgenic lines