Fig. 3
Viability of L2 heterozygotes is lowered in the absence of PKD. Viability of PKD26 homozygotes (labelled grey) in a L2 / + heterozygous background, compared to the doubly heterozygous siblings derived from a cross of L2 / CyO; PKD26/TM3 Sb males with virgins PKD26/ PKD26. Each genotype is expected with the same frequency; the PKD26 homozygotes however, reach only about 12% expectancy in the background of L2 (0.12). Center lines of BoxPlots display the median, box limits indicate the 25th and 75th percentiles as determined by R-software, whiskers extend 1.5 times the interquartile range. Three experiments were performed with 333 total number of flies. Statistical significance of probes was determined by ANOVA two-tailed test for multiple comparisons using Dunnett’s approach relative to the doubly heterozygous control, with raw p-values: p > 0.05 (not significant); * p < 0.05; ** p < 0.01; *** p < 0.001