Fig. 2
From: Detect accessible chromatin using ATAC-sequencing, from principle to applications
The major procedures of ATAC-seq. a Nuclei preparation: target cells are lysed in the lysis buffer to collect nuclei. b Transposase reaction: adding the Tn5 transposase to tag the genomic DNA. The green symbol represents “adapter 1” of the Tn5 transposase, while the red symbol represents “adapter 2” of theTn5 transposase. c PCR amplification: PCR primer-1 and -2 are used to generate library for sequencing. Primer-1 and -2 are two universal PCR primers, which capture fragments with special length and add barcodes appropriate for the next generation sequencing