First report of bicolour FISH of Berberis diaphana and B. soulieana reveals interspecific differences and co-localization of (AGGGTTT)3 and rDNA 5S in B. diaphana

Table 1 Karyotype and FISH analysis in B. diaphana

Chr. No.	Chr. length (μm)			Arm ratio	FISH results			Chr. type
Chr. No.	Long arm	Short arm	L^b + S^c	Arm ratio	(AGGGTTT)₃		5S rDNA	Chr. type
1	1.50 ± 0.03^a	1.25 ± 0.03	2.75 ± 0.00	1.20 ± 0.05	L	S	–	m^d
2	1.47 ± 0.01	1.25 ± 0.01	2.72 ± 0.00	1.17 ± 0.01	L	S	–	m
3	1.58 ± 0.04	0.97 ± 0.01	2.55 ± 0.03	1.62 ± 0.05	L	S	–	m
4	1.41 ± 0.01	1.08 ± 0.01	2.49 ± 0.00	1.31 ± 0.02	L	S	–	m
5	1.44 ± 0.02	0.95 ± 0.04	2.40 ± 0.05	1.51 ± 0.04	L	S	–	m
6	1.25 ± 0.01	1.15 ± 0.02	2.40 ± 0.03	1.09 ± 0.01	L	S	L	m
7	1.63 ± 0.02	0.65 ± 0.04	2.28 ± 0.06	2.49 ± 0.11	L	SA^e	–	sm^f
8	1.22 ± 0.03	1.05 ± 0.02	2.28 ± 0.00	1.17 ± 0.05	L	S	–	m
9	1.17 ± 0.00	1.09 ± 0.01	2.25 ± 0.01	1.07 ± 0.01	L	S	–	m
10	1.22 ± 0.05	0.95 ± 0.01	2.17 ± 0.04	1.28 ± 0.07	L	S	–	m
11	1.17 ± 0.00	0.99 ± 0.01	2.16 ± 0.01	1.18 ± 0.01	L	S	–	m
12	1.08 ± 0.00	1.03 ± 0.00	2.11 ± 0.00	1.06 ± 0.01	L	S	–	m
13	1.00 ± 0.00	0.89 ± 0.01	1.89 ± 0.01	1.12 ± 0.02	L	S	–	m
14	1.04 ± 0.01	0.78 ± 0.03	1.82 ± 0.04	1.33 ± 0.04	L	S	–	m

Note: ^aMean ± standard deviation, ^blong arm, ^cshort arm, ^dmetacentric, ^esatellite, and ^fsubmetacentric. The chromosome pair numbers correspond to the numbers in Fig. 2a

ISSN: 1601-5223