Fig. 2

Metaphase (a) and prometaphase (b, c) plates of B. soulieana visualized after FISH. In (a), (AGGGTTT)₃ signals are green colour labelled with 5′– FAM; and 5S rDNA signals are red colour labelled with 5′–TAMRA and indicated with arrows, while arrowheads indicate satellites. In (b), arrows indicate the 5S rDNA signals of chromosomes. In (c), arrows indicate secondary constrictions of the chromosomes with large 5S rDNA signals. Chromosome number in (d) of B. soulieane was sorted by length (captured from a)