Fig. 3

Hairless orthologs from Triops and Strigamia. a Alignment of predicted Hairless orthologs from Triops cancriformis (TrcaH) and Strigamia maritima (StmaH) with the Daphnia pulex (DapuH) ortholog. Daphnia and Triops Hairless share the characterized functional domains, the Su(H) binding domain SBD which is subdivided into NT and CT box, the Groucho binding domain GBD, and the CtBP binding domain CBD. Only NT box and CBD are clearly discernable in Strigamia Hairless. The CT box and the GBD may be present with weak conservation only (dashed lines). Notably, residues known to contact the Su(H) CTD in D. melanogaster are identical in all the species (*). High confidence NLS and NES sequences are boxed, those of low confidence are dotted. Red arrowheads indicates an intron shared by all three genes. Black and blue arrowheads depict intron positions specific to Strigamia and Triops Hairless genes, respectively. Identical residues are marked in blue, highly conserved in red, conserved in yellow. b Structure predictions of the Notch repressor complexes from Triops and Strigamia, respectively, using SWISS Model and template PDB ID: 5E24 of the Drosophila complex. Left CSL CTD and right Hairless interacting domain. Potentially interacting residues are highlighted in yellow in Hairless, and green and orange in CSL