Fig. 10

Conservation of Prickle like proteins. a Alignment of human Prickle homolog 2 (HS pk; GenBank AAI19003.1), Drosophila melanogaster Prickle isoform A (Dm pk; flybase) and Hydra vulgaris Prickle 3 like (Hv pk3l). All three share the PET domain and the first three LIM domains. In this part many residues are identical (red), blue residues are not conserved. The Hydra protein has three additional LIM domains, whereas the human and fly Prickle proteins extend C-terminally with no further similarities or discerned motifs. Reduced stringency conditions were used to allow alignment of LIM domain three. Notably, a tetra-peptide motif TMVP with high similarity to the Lmpt motif is shared by all Prickle proteins at the corresponding position (italic green) in the PET domain. Blue arrows indicate intron positions identical in all genes, green identical in human and Drosophila and red identical between human and Hydra. Black arrows and dashes present species specific introns. b Schematic comparison of Prickle (pk), Prickle 3 like (pk3l) and Limpet (Lmpt) proteins from human (Hs), Drosophila (Dm) and Hydra (Hv). PET and LIM domains are color coded green and yellow, respectively. Intron positions are indicated by dashes: purple conserved in Limpet and prickle, blue conserved between KyoT2 and Drosophila Limpet and grey conserved in prickle. Only conserved intron positions are shown. Positions of defined CID motif in KyoT2 (VWWP) and potential interacting motifs (TWVP like) in the others are indicated