Long-read sequencing identified a causal structural variant in an exome-negative case and enabled preimplantation genetic diagnosis

Table 2 Primers for Sanger sequencing. No.1–10: Exon primers; No.11–20: Quantitative real-time primers; No.21–26: Breakpoint primers

No.	Primer	Position	Sequence (5′-3′)
1	G6PC-1F	Exon1	CACCACCAAGCCTGGAATAAC
2	G6PC-1R	Exon1	CAGACATTGCGAGAGCGAATG
3	G6PC-2F	Exon2	GCATTCATTCAGTAACCC
4	G6PC-2R	Exon2	AGACAGAAGCTGAGTGGA
5	G6PC-3F	Exon3	CACCTTTACTCCATTCTCTTTC
6	G6PC-3R	Exon3	GTGCCACAACTCTTAATCAGCG
7	G6PC-4F	Exon4	CACTGAGAGCACCTAAGTTTGC
8	G6PC-4R	Exon4	CTGATTACACACAGGATGTGG
9	G6PC-5F	Exon5	CATGTCACCCACTCCTCCAAAC
10	G6PC-5R	Exon5	GTCACTTGCTCCAAATACCAGTG
11	G6PC-1ForF	5′-Flanking introns	TTTCACAGTCCTCCGTGACC
12	G6PC-1ForR	5′-Flanking introns	AGGGCTTCTATATCTTGAGCTTTC
13	G6PC-1QF	Exon1	TCCAGTCAACACATTACCTCCA
14	G6PC-1QR	Exon1	TAAAGACGAGGTTGAGCCAGTC
15	G6PC-2inF	Intron2–3	AAGTTGGGACAAGGGAATCAGA
16	G6PC-2inR	Intron2–3	CATTCTTAATTCCTCTACCCTGAGA
17	G6PC-4QF	Exon4	GCTGAAGGATCTGCACCTGT
18	G6PC-4QR	Exon4	AGGGAGTCAGATCAGCCCAT
19	G6PC-5QF	Exon5	CAGCTTCGCCATCGGATTTT
20	G6PC-5QR	Exon5	ACAATAGAGCTGAGGCGGAA
21	G6PC-D1F		GTGGGGAAAATGCCTGAGGA
22	G6PC-D2F		TTTTCACCCTTGGGAGCCTG
23	G6PC-D3F		GGTCACCCTGTCCCACTAGA
24	G6PC-D4F		CTCACCTGTTTTCCCACGGA
25	G6PC-D5F		GGGAGGAGACTCCAGGTCAT
26	G6PC-comR	Intron2–3	CTTTCCAGTCTGTGCCTCCAT