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Table 2 Primers for Sanger sequencing. No.1–10: Exon primers; No.11–20: Quantitative real-time primers; No.21–26: Breakpoint primers

From: Long-read sequencing identified a causal structural variant in an exome-negative case and enabled preimplantation genetic diagnosis

No.

Primer

Position

Sequence (5′-3′)

1

G6PC-1F

Exon1

CACCACCAAGCCTGGAATAAC

2

G6PC-1R

CAGACATTGCGAGAGCGAATG

3

G6PC-2F

Exon2

GCATTCATTCAGTAACCC

4

G6PC-2R

AGACAGAAGCTGAGTGGA

5

G6PC-3F

Exon3

CACCTTTACTCCATTCTCTTTC

6

G6PC-3R

GTGCCACAACTCTTAATCAGCG

7

G6PC-4F

Exon4

CACTGAGAGCACCTAAGTTTGC

8

G6PC-4R

CTGATTACACACAGGATGTGG

9

G6PC-5F

Exon5

CATGTCACCCACTCCTCCAAAC

10

G6PC-5R

GTCACTTGCTCCAAATACCAGTG

11

G6PC-1ForF

5′-Flanking introns

TTTCACAGTCCTCCGTGACC

12

G6PC-1ForR

AGGGCTTCTATATCTTGAGCTTTC

13

G6PC-1QF

Exon1

TCCAGTCAACACATTACCTCCA

14

G6PC-1QR

TAAAGACGAGGTTGAGCCAGTC

15

G6PC-2inF

Intron2–3

AAGTTGGGACAAGGGAATCAGA

16

G6PC-2inR

CATTCTTAATTCCTCTACCCTGAGA

17

G6PC-4QF

Exon4

GCTGAAGGATCTGCACCTGT

18

G6PC-4QR

AGGGAGTCAGATCAGCCCAT

19

G6PC-5QF

Exon5

CAGCTTCGCCATCGGATTTT

20

G6PC-5QR

ACAATAGAGCTGAGGCGGAA

21

G6PC-D1F

 

GTGGGGAAAATGCCTGAGGA

22

G6PC-D2F

 

TTTTCACCCTTGGGAGCCTG

23

G6PC-D3F

 

GGTCACCCTGTCCCACTAGA

24

G6PC-D4F

 

CTCACCTGTTTTCCCACGGA

25

G6PC-D5F

 

GGGAGGAGACTCCAGGTCAT

26

G6PC-comR

Intron2–3

CTTTCCAGTCTGTGCCTCCAT