Table 1 Primer sequence used in this study
From: Characterization of TaDREB1 in wheat genotypes with different seed germination under osmotic stress
Primer | Primer sequence(5′-3′) | Fragment size (bp) | Anneal temp.(°C) |
|---|---|---|---|
DREB1-A1F | CGGAACCACTCCCTCCATCTC | 1107 | 62 |
DREB1-A1R | CGGTTGCCCCATTAGACGTCA | ||
DREB1-A2F | CTGGCACCTCCATTGCTGAC | 599 | 63 |
DREB1-A2R | AGTACATGAACTCAACGCACAGGACAAC | ||
DREB1-BF | CCCAACCCAAGTGATAATAATCT | 716 | 58 |
DREB1-BR | TTGTGCTCCTCATGGGTACTT | ||
DREB1-D1F | TCGTCCCTCTTCTCGCTCCAT | 1190 | 63 |
DREB1-D1R | GCGGTTGCCCCATTAGACATCG | ||
DREB1-D2F | CTGGCACCTCCATTGCCGAT | 596 | 64 |
DREB1-D2R | AGTACATGAACTCAACGCACAGGACAAC | ||
DREB U | TCGTCCCTCTTCTCGCTCCATGG | 493 | 66 |
DREB D | GGGCATGGCG CCGCATGG | ||
DREB1-AF | ATGAACAGGAAGAAGAAAGTGCGC | 593 | 62 |
DREB1-AR | TTCTCAAATCATTGCTCACT TCTTTC | ||
DREB1-BF | ATGACCAGGAAGAAGAAAGTGCGC | 585 | 60 |
DREB1-BR | TCATTGCTCACTTCTTTTTTCACCTTAT | ||
DREB1-DF | ATGAACAGGAAGAAGAAAGTGCGC | 455 | 62 |
DREB1-DR | TCCTTCCCATCAGAAGGATGTGAC | ||
β-actin F | GTTTCCTGGAATTGCTGATCGCAT | 410 | 65 |
β-actin R | CATTATTTCATACAGCAGGCAAGC |