Molecular markers associated with aluminium tolerance in Sorghum bicolor

Hereditas

Table 2 Simple and multiple linear regression analysis of 229 F_2:3 progeny plants for the association between marker-based genotypes and net root length in aluminium (NRL_Al) and percent relative root growth (%RRG); and Chi-square goodness- of-fit test for Mendelian segregation of the markers

Marker	^dChromosome number	^dMarker position (bp range)	NRL_Al			%RRG			χ ² test
			F	P-value	R²	F	P-value	R²	χ²	P-value
Sb5_236	3	52,278,272–52,278,445	15.08	0.000	0.110	11.92	0.001	0.050	5.33	0.070
Xtxp34	3	69,704,047–69,704,411	18.75	0.000	0.142	17.07	0.000	0.070	3.94	0.139
CTG29_3b	3	70,939,651–70,939,834	11.42	0.001	0.048	9.49	0.002	0.040	3.60	0.059
811_1400 ^a	3	71,473,923–71,475,318	24.88	0.000	0.100	9.78	0.002	0.041	0.14	0.709
884_200 ^b	6	42,689,435–42,689,555	4.64	0.032	0.017	5.07	0.025	0.025	3.09	0.079
Sb6_342	7	38,823,193–38,823,478	4.49	0.012	0.038	6.06	0.015	0.030	0.36	0.837
Sb6_34	8	61,802,592–61,802,795	7.58	0.001	0.063	4.02	0.046	0.020	5.70	0.058
835_200	10	56,491,522–56,491,702	3.61	0.049	0.016	6.85	0.009	0.029	1.50	0.220
Four markers^c	3		15.67	0.000	0.219	7.06	0.000	0.119
All markers			8.15	0.000	0.251	3.82	0.000	0.122

^aCcorresponds to SCAR_811; ^bcorresponds to SCAR_884; ^cthe four markers on chromosome 3. ^dThe chromosome number and the position of the markers within the corresponding chromosomes (in bp range) were identified through BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) search of the sequences of the markers against the sorghum genome. The GenBank Accession number of chromosomes 3, 6, 7, 8 and 10 are NC_012872.2, NC_012875.2, NC_012876.2, NC_012877.2, NC_012879.2, respectively. Note: the position of SbMATE gene on chromosome 3 (NC_012872.2) is 71,105,461–71,108,054 bp

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