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Table 1 List of primers/primer-pairs used to amplify markers that showed association with Al tolerance in sorghum

From: Molecular markers associated with aluminium tolerance in Sorghum bicolor

Marker type

Locus name

Primer sequences (5′ - 3′)

Repeat motif

Fragment size range (bp)

Ta (°C)

ISSR

ISSR_811 a

F/R: GAG AGA GAG AGA GAG AC

1400

55

ISSR_835 a

F/R: AGA GAG AGA GAG AGA GCT C

Ca. 200

55

ISSR_884 a,i

F/R: HBH ATC AGA GAG AGA GAG AG

Ca. 200

55

SSR

Sb5_236 b

F: GCC AAG AGA AAC ACA AAC AA

R: AGC AAT GTA TTT AGG CAA CAC A

(AG)20

160–208

55

Sb6_342 b

F: TGC TTG TGA GAG TGC CTC CCT

R: GTG AAC CTG CTG CTT TAG TCG ATG

(AC)25

270–294

50

Sb6_34 b

F: AAC AGC AGT AAT GCC ACA C

R: TGA CTT GGT AGA GAA CTT GTC TTC

[(AC)/(CG)]15

188–208

55

Xtxp34 c

F: TGG TTC GTA TCC TTC TCT ACA G

R: CAT ATA CCT CCT CGT CGC TC

(CT)29

365

55

STS

CTG29 d,e

F: ATG CAG TAT CTG CAG TAT CAT TT

R: AAT CCG TCA GGT CAG CAA TC

226–228

57

M181 d,e

F: AAG GCA ACA ACT GAG GCA CT

R: TCG CTA GAG TGG TGC AAG AA

169–174

55

CTG29_3b f,g

F: TGG TGA TAT TAT TAA AAC TGT GTT A

R: AAT CCG TCA GGT CAG CAA TC

200

58

SCAR

SCAR_811 h

F: ACG CAA GTT CCG AGG AGA A

R: GAG AGA GAG AGA GAG ACA GAG GTT GTC

1119

65

SCAR_884 h

F: AGA GAG AGA GAG AGC TCA CAC A

R: AGA GAG AGA GAG AGG TGT TTT A

226 and 446

61

  1. aSource: University of British Columbia, Canada: http://www.scribd.com/doc/23812434/UBC
  2. bSource: Smith et al. [54], cSource: Kong et al. [55], dSource: Caniato et al. [23]; eThe markers did not differentiate the resistant and sensitive parents in this study. fSource of forward primer: this study; gSource of reverse primer: Caniato et al. [23]; hSource: this study. F = Forward; R = Reverse; F/R = used as both forward and reverse primers. iIn the primer sequence of ISSR_884, B = C or G or T whereas H = A or C or T