From: Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco
Primer sets | NCBI Accession No. for the target gene | Primer Sequence (5′-3′)) | Purpose |
---|---|---|---|
PvDREB1C_CDS | Pavir.J35991.1 | ATACGGATCATGGAGTACGACGAGCAGGAG | Cloning of PvDREB1C |
TGATAAGCTTGTCGTAGTAGCTCCACAGCGTC | |||
35S_F1 & GFP_R1 | – | TCCACTGACGTAAGGGATGAC | PCR verification of transgenic tobacco |
AGAATTGGGACAACTCCAGTG | |||
PvDREB1C_qRT | Pavir.J35991.1 | GGAGTACGACGAGCAGGAGT | qRT-PCR |
GGTCTCCCGGAACTTGGT | |||
NtSOD | AB093097 | AGCTACATGACGCCATTTCC | qRT-PCR |
CCCTGTAAAGCAGCACCTTC | |||
NtPOD | D11396.1 | GCTGTTCGACGAGTTGTTAACAG | qRT-PCR |
CTCTGGCTGAGTTGTTGTTGG | |||
NtAPX1 | AU15933 | CAAATGTAAGAGGAAACTCAGAGGA | qRT-PCR |
CAGCCTTGAGCCTCATGGTACCG | |||
NtcAPX | D85912 | CTGGAGTTGTTGCTGTTG | qRT-PCR |
GGTGGCTCTGTCTTGTC | |||
NtNHX4 | XM_016617731 | CAAGAACTTCCGCACCCAC | qRT-PCR |
GCAGTATCAAACGCAGAGGACC | |||
NtSOS1 | XM_016611194 | CAAATGTTATCCCCCGAAAGC | qRT-PCR |
CGGAGAACCTGAGGAAATGTGA | |||
NtERD10B | AB049336 | CAATTTAGTGCAGGCCAGGC | qRT-PCR |
GGTCCATGGTGGCCAGGAAG | |||
NtPAO | AB200262 | GCTGCTCTGTCGTCATA | qRT-PCR |
TATCCTGCCACCTATCTTATC | |||
NtCuAO1 | DQ873385 | TATGGCAGTTGATTGTAAGC | qRT-PCR |
TGACGAGCAGAGAATGG | |||
NtCuAO2 | AB289457 | TATGGCAGTTGATTGTAAGC | qRT-PCR |
CCAATGACGAGCAGATAAAGT | |||
NtUbiquitin | XM_016580871 | TCCAGGACAAGGAGGGTAT | qRT-PCR |
CATCAACAACAGGCAACCTAG |