Fig. 6
nbr ablation increases retro-element RNA intermediates. Semi quantitative RT-PCR of total RNA from ovaries of wild type females (Ow), transgenic RNAi UAS-nbr i 37/UAS-nbr i 37; tub-GAL4/TM3 Sb ovaries (Oi) and as a further control of total RNA of whole wild type adult females (Aw), assayed for the copia TE showing up as 438 bp fragment. PCR reactions were 31 and 34 cycles, as indicated at the bottom. Blue numbers above the bands indicate values of the optical intensity of the bands upon optical measurement, shown in arbitrary units. The RNA levels of the ribosomal protein 49 (rp49) gene were taken as internal control showing up as a 388 bp band, whose optical intensities are indicated as green numbers below the bands. The length of the target genes is indicated to the left