Fig. 5
nbr knockdown reduces the silencing effect of RNA interference. a and b Reduction of the silencing effect of nbr in S2 cells. a cells transfected with the reporter plasmid pAc-EGFP and RNAi against EGFP and mock RNAi. Expression of EGFP is completely abolished. b When mock RNA i is replaced with nbrRNA i, expression of the EGFP reporter gene is increased in a number of cells. c-f phenotypic classes of males flies expressing dsRNA against Drosophila dystroglycan (Dg, CG18250) related to the occurrence of the posterior cross vein (PVC) in wings, divided into 4 classes of decreasing severity: (c) <5%, (d) 5-50%, (e) 50-100% and (f) 100% = wild-type. In wings of Dg-knockdown flies, PVCs extending more than 50% are observed in ~53% of the wings (see Table 1). In wings of doubly Dg, nbr knockdown flies, this number is increased to ~73%, demonstrating that the RNAi activity of Dg is suppressed by lowered activity of nbr