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Fig. 3 | Hereditas

Fig. 3

From: Drosophila exoribonuclease nibbler is a tumor suppressor, acts within the RNAi machinery and is not enriched in the nuage during early oogenesis

Fig. 3

Cellular localization of Nbr and Western analysis. a a string of ovaries of different stages stained for Nbr (red) and the nuclear stain DAPI (blue). Nbr is expressed only in all 3 stages of the germarium and stage 2 oocytes. b-d super resolution picture of a stage 2 oocyte stained for Nbr (b), DAPI (c) and merge (d). e-g stage 10 oocyte stained for Nbr (e), DAPI (f) and merge (g). h-j staining of Nbr in embryos, nuclear cycle (nc) 6 embryo (h), nc 11 embryo (i) and nc 14 embryo (j). k Western analysis of wild-type 4-8 h extracts (left lane) and prd-GAL4 > UAS-nbr 4-8 h extracts (right lane) probed with the nbr antiserum. A single band of ~60 kD is observed which is upregulated upon over-expression using the prd-GAL4 driver (right lane)

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Hereditas

ISSN: 1601-5223

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