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Table 4 Mutations identified in recessive brh1 alleles and their deduced effect at protein level

From: Mutations in the gene of the Gα subunit of the heterotrimeric G protein are the cause for the brachytic1 semi-dwarf phenotype in barley and applicable for practical breeding

Allele

Position of mutationa

Type of mutation

Effect of mutation on protein

BW047 (ari-i.38)

2345

a to t

Mutation shifts splice site between intron 8 and exon 9 leading to frame shift. 203 native amino-acid residues followed by FSAAPLERAKEAERYTGCMM.

BW074 (brh1.a)

3745-3746

2 bp deletion

Frame shift eliminates wild type stop codon and extends exon 13. 372 native residues followed by 152 additional aa residues.

BW075 (brh1.aa)

2205

1 bp deletion

Frame shift in exon 8. 185 native residues followed by MCSMQEYGQMGL.

BW076 (brh1.ae)

Deletion (minimal region: −723 to 2819

Large deletion, no transcript detectable

Probably no protein produced

brh1.c

3745-3746

2 bp deletion

Frame shift eliminates wild type stop codon and extends exon 13. 372 native residues followed by 152 additional residues.

BW077 (brh1.e)

813

g to a

Mutation eliminates splice site between intron 4 and exon 5 leading to frameshift. 88 native residues followed by VCYYWKGCVSYLFPHLLHSGDYSGNLHWIFLWECVYTSYY.

brh1.f

1512-1515

4 bp deletion

Frame shift and premature stop in exon 6. 129 native residues followed by SLIKNSYRM.

BW078 (brh1.t)

2346

g to a

Mutation shifts splice site between intron 8 and exon 9. 203 native residues followed by FSATPLERAKEVERYTGCMM.

BW079 (brh1.x)

3718

a to t

Premature stop in exon 13. Truncated protein of 363 residues.

BW080 (brh1.z)

2205

1 bp deletion

Frame shift in exon 8. 185 native residues followed by MCSMQEYGQMGL.

ari-m.12

3178

g to a

Premature stop in exon 10. Truncated protein of 270 native residues.

BW051 (ari-m.28)

3178

g to a

Premature stop in exon 10. Truncated protein of 270 native residues.

ari-m.141

164

g to a

Mutation eliminates splice site between intron 1 and exon 2. 21 native residues followed by VSSFPYHLLDSFNSSCPVLSCPVLS.

ari-m.177

2345

a to t

Mutation shifts splice site between intron 8 and exon 9 leading to frame shift. 203 native residues followed by FSAAPLERAKEAERYTGCMM.

ari-m.251

 

Large deletion

No protein produced.

ari-m.269

3449

g to a

Premature stop in exon 12. Truncated protein of 306 residues.

  1. The deduced polypeptide of Brh1 (AF267485) is 383 amino-acid residues
  2. aFirst position is the “A” of the ATG start codon in the Bowman genomic sequence