Table 2 Oligonucleotides used as PCR primers to amplify and sequence the gene encoding the Gα-subunit of the heterotrimeric G protein and to amplify gene fragments located in the genomic region surrounding this gene in a BAC pool screening
Primer name | Sequence (5′ → 3′) | Template | Length of amplification product [bp] |
|---|---|---|---|
bw-Fr1-F1 | TGTCAAGATGATGGCTTCAG | Genomic DNA | 703 (with bw-Fr1-R1) |
bw_Fr1-Fseq | AAAAAGACGATGATGAAGC | Genomic DNA | |
bw-Fr1-R1 | GATCGACGAGAGCATGAGAC | Genomic DNA | |
bw-Fr2-F1 | GGCGAGGTAGAAAGCAAAAG | Genomic DNA | 790 (with bw-Fr2-R1) |
bw-Fr2-R1 | TTTTGAGGTATCCATCCATCTC | Genomic DNA | |
bw-Fr3-F1 | CGCACACAGTCAAAGGAAC | Genomic DNA | 896 (with bw-Fr3-R1) |
bw-Fr3-R1 | GACAGCATTGCACAAGGAG | Genomic DNA | |
bw-Fr4-F1 | TGTCCCAGATCCTCAAACTG | Genomic DNA | 901 (with bw-Fr4-R1) |
bw-Fr4-R1 | TTGTCCCTTGTAAACTGTTGG | Genomic DNA | |
bw-Fr5-F1 | TCCTCCTGCAAAATCTCTCC | Genomic DNA | 903 (with bw-Fr5-R1) |
bw-Fr5-R1 | TTAGACTCGGCATTTTGAGG | Genomic DNA | |
bw-Fr6-F1 | ACCTGAATGGCTGGATCTTC | Genomic DNA | 857 (with bw-Fr6-R1) |
bw-Fr6-R1 | GCATAGTGGGGATTATTCAGG | Genomic DNA | |
bw-Fr7-F1 | GGTCCAAACAGGTTCAGTTG | Genomic DNA | 824 (with bw-Fr7-R1) |
bw-Fr7-R1 | GATTCTGCACGAGACAAAGG | Genomic DNA | |
bw-Fr8-F1 | GGAATCAGTCTTTCCAGATCC | Genomic DNA | 705 (with bw-Fr8-R2) |
bw-Fr8-R2 | CCAAAATACCCATACCAACC | Genomic DNA | |
bw-Fr9-F1 | GAGCGAGCCAGAGATTTTG | Genomic DNA | 711 (with bw-Fr9-R1) |
bw-Fr9-R1 | TGTATATGACGGAGCAGCAAG | Genomic DNA | |
bw_Fr9-Rseq | GCTTGTTGGTTGTAGCTCAG | Genomic DNA | |
IL130 | CAACAATATGGGCATTACAT | BAC | 350 (with IL131) |
IL131 | CGAGGAGTTCTACAAATCAT | BAC | |
IL132 | CTTGCCCACTTACCAGGTGG | BAC | 300 (with IL133) |
IL133 | ACGGGATCTCCTTATGGAGC | BAC | |
IL134 | ATGATCGACTGCTACATCC | BAC | 318 (with IL135) |
IL135 | GGTTCTGTCGTTGTACCTAG | BAC | |
IL136 | CACCAAGCAGCAGATGATC | BAC | 152 (with IL137) |
IL137 | CCAGTTTGTTGGATGTTTCC | BAC | |
IL138 | GAGGGCTTCTATTGAAAGTG | BAC | 510 (with IL139) |
IL139 | GGTACTGACCAACTGTTACG | BAC | |
IL140 | AAAAGGCTGACTCAAAGACC | BAC | 318 (with IL141) |
IL141 | CGACAAGCTCCATAAGGTAC | BAC | |
IL142 | TCTGACCCAGAAACTTGATC | BAC | 651 (with IL143) |
IL143 | GAAAGGGAGAGAGAAATCG | BAC | |
IL144 | GGTCTGACCTCTTATTTCCC | BAC | 512 (with IL145) |
IL145 | AAAGCGAGGCATTCACTAG | BAC | |
IL146 | GCTCAAAGTGGGAATACGAG | BAC | 475 (with IL147) |
IL147 | CTTGTGCTGCCAACTAGATG | BAC | |
IL148 | AAGAGCATCCAGTGACAAG | BAC | 633 (with IL149) |
IL149 | GGATCTCCGCTTAGGTATAG | BAC | |
IL150 | CGCTTTCCCTATTTTGTCAG | BAC | 576 (with IL151) |
IL151 | ACTTCACGGAGATTGATGC | BAC | |
IL152 | AGCCAGCAATCCTTGTTAGC | BAC | 510 (with IL153) |
IL153 | ACCTTGAGCCAGGGAAAAG | BAC | |
IL201 | CTCTGGCTCGCTCATCAACC | RNA | 1229 (with IL202) |
IL202 | AGCGTCTCATGCTCTCGTCG | RNA | |
IL203 | GTAAACGAGGCAGAGGCAGC | RNA | 461 (with IL204) |
IL204 | CAGTCGGGAACTTGCAGCAC | RNA | |
IL205 | CGGAACTCAAGGGCTACACG | RNA | 600 (with IL206) |
IL206 | CGAACAGCTCCTTGGTCTCC | RNA | |
IL207 | AAATCTCTGGCTCGCTCATC | RNA | 1389 (with IL208) |
IL208 | AGCACAAAGGAGTCACAAGG | RNA | |
IL209 | ACTAGGAGGAAGCATGAGAC | RNA | 1305 (with IL210) |
IL210 | CAATACAGAGTTGCGACACC | RNA |