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Table 2 List of SSR primers used for genotyping of 75 soyabean genotypes along with their product size, no. of alleles amplified, gene diversity, heterozygosity and PIC value

From: Genetic diversity and population structure analysis of Kala bhat (Glycine max (L.) Merrill) genotypes using SSR markers

Marker

Size (bp)

Forward primer

Reverse primer

Major allele frequency

Allele No

Gene diversity

Heterozygosity

PIC

sat005

141

TATCCTAGAGAAGAACTAAAAAA

GTCGATTAGGCTTGAAATA

0.6000

2.0000

0.4800

0.0571

0.3648

sat385

310

AATCGAGGATTCACTTGAT

CATTGGGCCACACAACAAC

0.6081

2.0000

0.4766

0.0000

0.3630

sat415

297

GCGTCTCCCTTAATCTTCAAGC

GCGTGTGACGGTTCAAAATGATAGTT

0.6197

3.0000

0.4999

0.0000

0.4104

sat577

119

CAAGCTTAAGTCTTGGTCTTCTCT

GGCCTGACCCAAAACTAAGGGAAGTG

0.6884

3.0000

0.4637

0.0145

0.4039

sat180

242

TCGCGTTTGTCAGC

TTGATTGAAACCCAACTA

0.3542

4.0000

0.7210

0.1250

0.6689

sat277

243

GGTGGTGGCGGGTTACTATTACT

CCACGCTTCAGTTGATTCTTACA

0.6923

2.0000

0.4260

0.0000

0.3353

sat422

250

ATTAGGGGAGGGGAGGTAAAAAGT

TGAAGGCCCGATATCCAAATAAA

0.5208

3.0000

0.5651

0.0139

0.4742

sat600

195

GCGCAGGAAAAAAAAACGCTTTTATT

GCGCAATCCACTAGGTGTTAAT

0.5625

4.0000

0.6189

0.1389

0.5753

sat389

232

GCGGCTGGTGTATGGTGAAATCA

GCGCCAAAACCAAAAGTTATATC

0.9400

2.0000

0.1128

0.0400

0.1064

sat411

97

TGGCCATGTCAAACCATAACAACA

GCGTTGAAGCCGCCTACAAATATAAT

0.5462

2.0000

0.4957

0.0154

0.3729

sat554

261

GCGATATGCTTTGTAAGAAAATTA

GCGCAAGCCCAAATATTACAAATT

0.5530

4.0000

0.5874

0.0758

0.5200

sat285

236

GCGACATATTGCATTAAAAACATACTT

GCGGACTAATTCTATTTTACACCAACAAC

0.9400

2.0000

0.1128

0.0133

0.1064

sat183

240

TAGGTCCCAGAATTTCATTG

CACCAACCAGCACAAAA

0.6800

2.0000

0.4352

0.0000

0.3405

sat431

250

GCGTGGCACCCTTGATAAATAA

GCGCACGAAAGTTTTTCTGTAACA

0.4857

3.0000

0.6171

0.0571

0.5409

sat247

221

GCGCCCATGTGGCTATTTCTTTATTT

GCGGATCAATAATAAACAAAGTGACAA

0.8933

2.0000

0.1906

0.0000

0.1724

sat175

163

GACCTCGCTCTCTGTTTCTCAT

GGTGACCACCCCTATTCCTTAT

0.8733

2.0000

0.2212

0.0133

0.1968

sat306

212

GCGCTTAAGGACACGGATGTAAC

GCGTCTCTTTCGATTGTTCTATTAG

0.5074

2.0000

0.4999

0.9853

0.3749

sat255

141

GCGCTTTTAGCGTCGTCTGGC

TACCCCTCTCTTATTCTTCTT

0.8493

3.0000

0.2597

0.0274

0.2324

sat584

189

GCGCCCAAACCTATTAAGGTATGAACA

GCGGGTCAGAAGATGCTACCAAACTCT

0.7719

2.0000

0.3521

0.0000

0.2901

sat420

232

GCGTATTCAGCAAAAAAATATCAA

TTATCGCACGTGTAAGGAGACAAAT

0.7800

2.0000

0.3432

0.0133

0.2843

sat478

190

CAGCCAAGCAAAAGATAAATAATA

TCCCCCACAAGAGAACAAGAAGGT

0.5423

4.0000

0.6341

0.8592

0.5886

   

Mean

0.6671

2.6190

0.4340

0.1166

0.3677

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Hereditas

ISSN: 1601-5223

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