Table 7 Estimation of development and running costs for (d)CAPS, tetra marker and CEL-I-based genotyping (in US$)
From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean
Development costs | |||
|---|---|---|---|
| Â | CAPS (dCAPS) | Tetra marker | CEL-I |
Primer | 7.5 | 15 | 7.5 |
Restriction enzyme | 30 | Â | 1 |
PCR reagents | 0.48 | 0.48 | 0.96 |
Gel electrophoresis and DNA band detection | 0.06 | 0.06 | 0.12 |
TOTAL | 38.04 | 15.54 | 9.58 |
Work load | |||
 Approximate working time for development 1 assay (h) | 0.25 | 0.25 | 0.1 |
 Working time for testing | 0.12 | 0.08 | 0.12 |
TOTAL | 0.37 | 0.33 | 0.22 |
Routine analysis | |||
 Hands-on time per assay (assuming 96 assays per run, in h) | 0.03 | 0.025 | 0.06 |
 Reagent costs per assay | 0.13 | 0.105 | 0.19 |