Table 7 Estimation of development and running costs for (d)CAPS, tetra marker and CEL-I-based genotyping (in US$)
From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean
| Development costs | |||
|---|---|---|---|
| CAPS (dCAPS) | Tetra marker | CEL-I | |
| Primer | 7.5 | 15 | 7.5 |
| Restriction enzyme | 30 | 1 | |
| PCR reagents | 0.48 | 0.48 | 0.96 |
| Gel electrophoresis and DNA band detection | 0.06 | 0.06 | 0.12 |
| TOTAL | 38.04 | 15.54 | 9.58 |
| Work load | |||
| Approximate working time for development 1 assay (h) | 0.25 | 0.25 | 0.1 |
| Working time for testing | 0.12 | 0.08 | 0.12 |
| TOTAL | 0.37 | 0.33 | 0.22 |
| Routine analysis | |||
| Hands-on time per assay (assuming 96 assays per run, in h) | 0.03 | 0.025 | 0.06 |
| Reagent costs per assay | 0.13 | 0.105 | 0.19 |