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Table 7 Estimation of development and running costs for (d)CAPS, tetra marker and CEL-I-based genotyping (in US$)

From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean

Development costs
  CAPS (dCAPS) Tetra marker CEL-I
Primer 7.5 15 7.5
Restriction enzyme 30   1
PCR reagents 0.48 0.48 0.96
Gel electrophoresis and DNA band detection 0.06 0.06 0.12
TOTAL 38.04 15.54 9.58
Work load
 Approximate working time for development 1 assay (h) 0.25 0.25 0.1
 Working time for testing 0.12 0.08 0.12
TOTAL 0.37 0.33 0.22
Routine analysis
 Hands-on time per assay (assuming 96 assays per run, in h) 0.03 0.025 0.06
 Reagent costs per assay 0.13 0.105 0.19