Table 6 Mungbean SNPs found by Sanger sequencing of PCR fragments produced with the outer tetra marker primers. These SNPs are located nearby the SNPs that were detected by genotyping by sequencing
From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean
Locus name | Primer name | NM92 | TC1966 | NM94 | V2802 |
|---|---|---|---|---|---|
1:26,370,595 | tetra_6 | 60:K, 65:K, 94: V, 95:G | 64:T, 95:A | none | none |
2:23,741,639 | tetra_7 | 44:A, 127:T, 181: W | 44:T, 127: C | 127: C | 127: C, |
3:11,561,441 | tetra_8 | 56: M, 71:Y, 109:W | none | none | none |
3:10,830,938 | tetra_9 | 107:A, 117:G | 107:G, 117:A | 86:A | 86:T |
5:9,090,455 | tetra_10 | 71:T, 77:T, 95:A, 118:G, 138:C, 213:Y, | 71:C, 77:Y, 95:S, 118:K, 138:Y, 213:C, | 71:Y, 77:Y, 95:A, 102:W, 149:S, 178:S, 213:Y, 225:C | 24:C, 71:Y, 77:Y, 168:W, 178:S |
7:13,713,780 | tetra_11 | none | none | none | none |
10:3,159,416 | tetra_12 | none | none | none | none |
12:9,262,432 | tetra_13 | 81:C, 93:T | 81:T, 93:C | none | none |