Skip to main content

Table 6 Mungbean SNPs found by Sanger sequencing of PCR fragments produced with the outer tetra marker primers. These SNPs are located nearby the SNPs that were detected by genotyping by sequencing

From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean

Locus name Primer name NM92 TC1966 NM94 V2802
1:26,370,595 tetra_6 60:K, 65:K, 94: V, 95:G 64:T, 95:A none none
2:23,741,639 tetra_7 44:A, 127:T, 181: W 44:T, 127: C 127: C 127: C,
3:11,561,441 tetra_8 56: M, 71:Y, 109:W none none none
3:10,830,938 tetra_9 107:A, 117:G 107:G, 117:A 86:A 86:T
5:9,090,455 tetra_10 71:T, 77:T, 95:A, 118:G, 138:C, 213:Y, 71:C, 77:Y, 95:S, 118:K, 138:Y, 213:C, 71:Y, 77:Y, 95:A, 102:W, 149:S, 178:S, 213:Y, 225:C 24:C, 71:Y, 77:Y, 168:W, 178:S
7:13,713,780 tetra_11 none none none none
10:3,159,416 tetra_12 none none none none
12:9,262,432 tetra_13 81:C, 93:T 81:T, 93:C none none