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Table 5 Mungbean SNPs detected by genotyping by sequencing among the mapping parents NM92, NM94, TC1966 and V2802 and targeted for genotyping by tetra markers and CEL-I

From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean

GBS data

Sequencing data

Locus name

Primer name

SNP Position in PCR fragment

NM92

TC1966

NM94

V2802

NM92

TC1966

NM94

V2802

1:26,370,595

tetra_6

144

T

A

T

T

T

A

T

T

2:23,741,639

tetra_7

181

T

T

T

A

T

T

T

A

3:11,561,441

tetra_8

-

C

G

C

C

C

C

c

c

3:10,830,938

tetra_9

200

G

G

G

A

G

G

G

A

5:9,090,455

tetra_10

-

G

A

G

G

G,

G

G

G

7:13,713,780

tetra_11

-

A

A

A

G

A

A

A

A

10:3,159,416

tetra_12

171

C

G

C

G

C

G

C

G

12:9,262,432

tetra_13

140

T

C

C

C

T

C

C

C

  1. The numbers refer to the position of the SNP in the PCR fragment obtained from the mapping parents using the outer tetra primers. SNPs that could not be validated by sequencing are labeled in bold