Table 3 Allele-specific primers for rice
From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean
Locus name | Primer name | Fragment length | Common primer | Allele-specific primer 1 | Allele-specific primer 2 |
|---|---|---|---|---|---|
4:22435296 | AS1 | 219 | CGACAGGGAGAAATCCTGAA | 5’-GACTTGGCAGGTAAAACAGGTTACG-3' | 5’-CACTACAAAGGCTGTGGCAGGTAAAACAGGATAGA-3' |
5:44806 | AS2 | 189 | GCCTTGCAGGCTCCTATTTT | 5’-AGTCAAAGCTTCAGGCTCTAGCTGC-3’ | 5’-CGACTAACTGTGACTAAGCTTCAGGCTCTAGCATA-3’ |
6:14504992 | AS3 | 209 | CCTTGCCCTGTTATTCCTGA | 5’-CCAGTTTTCTCCTTGTGCCAGATTG-3’ | 5’-GATGTCTACTCCAGTTTTCTCCTTGTGCCAGAGAA-3’ |
7:3650191 | AS4 | 293 | CAAGTTTGTAGGTGCTGACCA | 5’-GATCCTAACTGAAGTGAAAACAGCA3’ | 5’-TGCGTCATAGGATCCTAACTGAAGTGAAAACACAC-3’ |
9:6982338 | AS5 | 162 | TTCTTGGGGTTGGCTACAAG | 5’-ACTGCTGTACTTGTATTTGCCGCAC-3’ | 5’-CATTGTATTTTTACGTGTACTTGTATTTGCCGATT-3 |