Table 2 Outer and inner primer for tetra markers for mungbean. The outer primer were also used for producing PCR fragments for CEL-I genotyping. The fragment length refers to the fragments produced with the outer primers
From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean
Locus name | Primer name | Frag. length | Outer Fwd | Inner Fdw | Inner Rev | Outer Rev |
|---|---|---|---|---|---|---|
1:26,370,595 | Tetra_6 | 208 | CCGAAGATGTGTGATTCATG | GACGATACTTGTCCAGATAT | GAAGGGATTTTGTTAGGAGT | AGCACTCAAGATGAAAGTGATC |
2:23,741,639 | Tetra_7 | 260 | ACTATCTGACCGAAAGGAA | TTGGTACCAAATTCTGCACT | ATCTTACGGTGAAGGACATT | ATCTGCTGACAGGAGAATTCA |
3:11,561,441 | Tetra_8 | 236 | CCCCTAAAGCTGGAATATA | TCTTCAAAGAGCTCCTTTTG | GATTGTCACTTTGTCAAAG | TGATCATCAAGCTACACTTC |
3:10,830,938 | Tetra_9 | 275 | AGCGAGGACAAGGAAGAATT | TCTGCGGTGTTCTTAGAGT | TTCCTCCAAATGCTGCAGAC | GGCAAATTATTAGGATCCTGC |
5:9,090,455 | Tetra_10 | 250 | TCGCTTAACCAAGAATCGC | CCAATGCGAATCAGATG | AAAACGCACATCTGGTTCGTGT | CGACATGGTGGCAATTTGAT |
7:13,713,780 | Tetra_11 | 253 | TAGCTGGTCCGTGTACTTTA | TTTCCATTGTGGGTCGTGGAGT | GCAGCAACATGTGCAACAT | GGAACTATGCTTTGGGACTT |
10:3,159,416 | Tetra_12 | 269 | ATACTGGAGGGTTGTTTCTA | TAAGCGTGCGCAGCCATAAACAAG | GGGTTTTTTCGGAAATTCAAAG | CGGTCTCAGAATCATAGTCTTG |
12:9,262,432 | Tetra_13 | 215 | AACCCCTTTATATAGGGTCTG | TCATCTTCTCCGGGCAGCTCCTTC | GAGGTGAAGGCACGTGACCA | TCATTCGAGGAAAGGAGAGA |