Table 1 Outer and inner primer for tetra markers for rice. The outer primers were also used for producing PCR fragments for CEL-I genotyping. The fragment length refers to the fragments produced with the outer primers
From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean
Locus name | Primer name | Frag. length | Outer Fwd | Inner Fdw | Inner Rev | Outer Rev |
|---|---|---|---|---|---|---|
4:22435296 | Tetra_1 | 310 | GGAACATGTCGCATTCACAGT | GCAGGTAAAACAGGTTAGG | AATTGATGCCTGACTGGCTGACT | CGACAGGGAGAAATCCTGAA |
5:44806 | Tetra_2 | 302 | ACACATCGATCCAGCTTACCA | TAAGCTTCAGGCTCTAGCTTC | GTGAGAAAGTAAAGGAC | GCCTTGCAGGCTCCTATTTT |
6:14504992 | Tetra_3 | 288 | CGCCAAGAAAATGTGCAGTA | TTCTCCTTGTGCCAGATAG | CACCACGGAGGTTTCTAAT | CCTTGCCCTGTTATTCCTGA |
7:3650191 | Tetra_4 | 222 | AGTGCCTGCATGATTGAACA | CTGAAGTGAAAACAGAGC | GTTTATTTCAAACGGGCG | CAAGTTTGTAGGTGCTGACCA |
9:6982338 | Tetra_5 | 201 | GCGGAATTACACTGTTTTTGG | TGTACTTGTATTTGCCGCTC | CCCTAAGATGTCTTAAAAATA | TTCTTGGGGTTGGCTACAAG |