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Fig. 6 | Hereditas

Fig. 6

From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean

Fig. 6

CEL-I test genotyping on pure and mixed DNA of 4 mungbean mapping parents for tetra_6 (a), tetra_7 (b), tetra_8 (c), tetra_9 (d), tetra_10 (e), tetra_11 (f), tetra_12 (g), and tetra_13 (h). CEL-I cuts at mismatch sites. Mismatches can appear at heterozygote sites in one genotype (as in NM92 with tetra_6 (a) in positions 60/65 and 94, or at SNP sites when PCR fragments from homozygote mapping parents are mixed, as in tetra_6 at position 144 of TC1966/NM92, or in tetra_12 (g). The SNPs among the mapping parents and the heterozygote loci causing the CEL-I fragments are labeled on the figures and listed in TablesĀ 5 and 6

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