Fig. 1
From: PCR-based assays for validation of single nucleotide polymorphism markers in rice and mungbean
Optimization of the incubation temperature for CEL-I treatment. The SNP tested was tetra_12. Incubation at 36 ° was found to be optimal to specifically cut at the mismatch site in position 171 bp of the 269 bp fragment, producing bands with 170 and 99 bp. The additional smaller band below around 90 bp is probably due to the sequence context around the SNP (please refer to the discussion)