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Table 3 Phytoene, lycopene, β-carotene, and chlorophyll content in F2 generation plants. The carotenoid pigments were quantified using HPLC (μg/g fresh weight) (n = 3, ± s.e.m) during mature (M), turning (T), and ripe (R) stages of ripening

From: Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation

Selected plant in F2 generation

Stage

Phytoene

Lycopene

β-carotene

Chlorophyll (a+b)

Red

M

0.01±0.14

0.04±0.12

0.91±0.61

2.84±0.17

T

0.46±0.42

14.03±0.02

2.97±0.35

0.14±0.38

R

1.68±0.21

32.12±0.64

4.03±0.64

0.08±0.12

Orange

M

0.01±0.34

0.02±0.07

1.16±0.28

2.54±0.16

T

0.18±0.13

0.16±0.27

7.94±2.32*

0.22±0.03

R

0.21±0.15

0.58±0.45

11.07±1.61*

0.07±0.07

Brown

M

0.01±0.03

0.01±0.03

1.12±0.26

5.84±0.12

T

0.24±0.02

8.27±0.28

1.84±0.17

2.97±1.06*

R

0.56±0.14

27.64±0.17

2.62±0.13

2.05±0.57*

Orange-brown

M

0.01±0.07

0.13±0.16

1.03±0.57

3.27±0.16

T

0.12±0.17

0.34±0.28

10.97±1.38*

2.68±0.26*

R

0.18±0.03

0.46±0.31

11.74±2.28*

2.03±0.68*

  1. Asterisk indicates phenotypes of which levels at same stages were significantly different than in the other phenotypes using a Tukey Pairwise Comparisons test (p<0.05)