Fig. 8

Quantitative real-time PCR validation of RNA-Seq data. Relative expression profiles of 30 genes showed the expression fold changes (FC) in comparison between the three fruit developmental stages in the black and white spine grapes. Histograms represent expression levels as assessed by RNA-Seq, data are reported as means ± SE of two biological replicates (left axis), the columns with diagonal lines or blank represent the RPKM of black or white spine grape, respectively. The line charts represent expression fold changes as assessed by qRT-PCR, data are reported as means ± SE of three replicates (right axis), the lines with black triangle or ‘×’ mark represent black or white spine grape, respectively