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Table 2 REMAP primers that were used in the analysis of timothy diversity, their sequences and properties

From: Assessment of genetic diversity in Nordic timothy (Phleum pratense L.)

Name

Sequence

nt

Tm (°C)

CG %

Linguistic complexity (%)

TIM1

GGTGCCGGCATCGATCCTTTCA

22

62.4

59.1

88

TIM2

ACGAGTGAGGACAAAGTGCGCAGA

24

61.9

54.2

79

ISSR1

ACCACCACCACCACCACCC

19

63.2

68.4

24

ISSR5

AGCAGCAGCAGCAGCAGCG

19

64.4

68.4

30

ISSR15

GTGGTGGTGGTGGTGGTGGTGA

22

64.2

63.6

28

ISSR20

TGCTGCTGCTGCTGCTGCC

19

64.6

68.4

30

  1. nt nucleotides, Tm melting temperature, CG % percentage of C and G bases