Table 1 The ISSR, SCoT, and EST-SSR primers used in this study and amplification results
Primer | Primer sequence (5′ → 3′) | Annealing (°C) | Total number of amplified bands (TNB) | The number of polymorphic bands (NPB) | Percentage of polymorphic bands (PPB) %) |
|---|---|---|---|---|---|
ISSR-UBC812 | (GA)8A | 52.0 | 13 | 12 | 92.31 |
ISSR-UBC827 | (AC)8G | 53.0 | 10 | 10 | 100.00 |
ISSR-UBC828 | (TG)8A | 52.0 | 9 | 8 | 88.89 |
ISSR-UBC829 | (TG)8C | 52.0 | 12 | 10 | 83.33 |
ISSR-UBC830 | (TG)8G | 55.0 | 14 | 13 | 92.86 |
ISSR-UBC835 | (AG)8YC | 52.0 | 15 | 13 | 86.67 |
ISSR-UBC836 | (AG)8YT | 54.0 | 17 | 14 | 82.35 |
ISSR-UBC844 | (CT)8RC | 52.0 | 14 | 12 | 85.71 |
ISSR-UBC848 | (CA)8RG | 53.0 | 14 | 13 | 92.86 |
ISSR-UBC854 | (TC)8RG | 52.0 | 15 | 15 | 100.00 |
ISSR-UBC868 | (GAA)6 | 55.0 | 13 | 12 | 92.31 |
ISSR-UBC876 | (GATA)2(GACA)2 | 52.0 | 16 | 13 | 81.25 |
ISSR-UBC879 | (CTTCA)3 | 53.0 | 17 | 14 | 82.35 |
ISSR-UBC887 | DVD(TC)7 | 52.0 | 14 | 14 | 100.00 |
ISSR-UBC890 | VHV(GT)7 | 52.0 | 13 | 11 | 84.62 |
ISSR-UBC891 | HVH(TG)7 | 52.0 | 14 | 12 | 85.71 |
SCoT2 | CAACAATGGCTACCACCC | 55.0 | 17 | 14 | 82.35 |
SCoT3 | CAACAATGGCTACCACCG | 55.0 | 34 | 31 | 91.18 |
SCoT4 | CAACAATGGCTACCACCT | 55.0 | 21 | 19 | 90.48 |
SCoT5 | CAACAATGGCTACCACGA | 55.0 | 21 | 19 | 90.48 |
SCoT6 | CAACAATGGCTACCACGC | 55.0 | 22 | 20 | 91.91 |
SCoT7 | CAACAATGGCTACCACGG | 55.0 | 22 | 20 | 91.91 |
SCoT9 | CAACAATGGCTACCAGCA | 55.0 | 20 | 19 | 95.00 |
SCoT10 | CAACAATGGCTACCAGCC | 55.0 | 21 | 20 | 95.00 |
SCoT12 | ACGACATGGCGACCAACG | 55.0 | 25 | 24 | 96.00 |
SCoT13 | ACGACATGGCGACCATCG | 55.0 | 25 | 22 | 88.00 |
SCoT15 | ACGACATGGCGACCGCGA | 55.0 | 18 | 16 | 88.89 |
SCoT16 | ACCATGGCTACCACCGAC | 55.0 | 26 | 24 | 92.31 |
SCoT18 | ACCATGGCTACCACCGCC | 55.0 | 20 | 18 | 90.00 |
SCoT21 | ACGACATGGCGACCCACA | 55.0 | 21 | 19 | 90.48 |
SCoT28 | CCATGGCTACCACCGCCA | 55.0 | 25 | 22 | 88.00 |
SCoT31 | CCATGGCTACCACCGCCT | 55.0 | 22 | 19 | 86.36 |
SCoT34 | ACCATGGCTACCACCGCA | 55.0 | 21 | 19 | 90.48 |
SCoT35 | CATGGCTACCACCGGCCC | 55.0 | 21 | 19 | 90.48 |
SCoT37 | CAATGGCTACCACTAGCC | 55.0 | 23 | 20 | 86.96 |
SCoT48 | ACAATGGCTACCACTGGC | 55.0 | 20 | 18 | 90.00 |
EST-SSR-cnl35 | f: AAGTGAGCACAACGACACGA | 58.0 | 9 | 8 | 88.89 |
r:CGATCCAAAGAAGCAAAGATG | |||||
EST-SSR-cnl37 | f:CTGCCTCGCGTGAAAGATA | 59.0 | 10 | 9 | 90.00 |
r:CCTCCTCGATCTGGATGGT | |||||
EST-SSR-cnl42 | f:GTTGGTCTGCTGCTCACTCG | 59.0 | 9 | 8 | 88.89 |
r:CCGACGATGTTGAAGGAGAG | |||||
EST-SSR-cnl47 | f: GACTCGCACGATTTCTCCTC | 57.0 | 9 | 8 | 88.89 |
r:GCCAGACAACCAATTCAGGT | |||||
EST-SSR-cnl51 | f:CTAGGGTTTCCCACCTCTCA | 59.0 | 8 | 6 | 75.00 |
r:AATGTCCTTGGCGTTGCT | |||||
EST-SSR-cnl55 | f:GCTGATAGCGAGGTGGGTAG | 58.0 | 14 | 11 | 78.57 |
r:CTGCCGGTTGATCTTGTTCT | |||||
EST-SSR-cnl61 | f:CACGAGTGCAGAGCTAGACG | 60.0 | 5 | 4 | 80.00 |
r:ACAACAACCCGACTGCTACC | |||||
EST-SSR-cnl86 | f:CAACAACGTCAACGCCTTC | 59.0 | 11 | 8 | 72.73 |
r:GCGTCTTGAACCTCTTGTCC | |||||
EST-SSR-cnl100 | f:CGTCGTCCTCTGCTGTGAG | 58.0 | 5 | 4 | 80.00 |
r:AGGTCGTCCATCTGCTGCT | |||||
EST-SSR-cnl115 | f:CGAGAAGAAGGTGGTGTCGT | 59.0 | 7 | 6 | 85.71 |
r:AGGTCGTGGAAGGTCTTGG | |||||
EST-SSR-cnl119 | f:ATCGTCTCCTCCTCCTCCA | 57.0 | 6 | 6 | 100.00 |
r:ATGCCTCGGTGGACTGGTA | |||||
EST-SSR-cnl130 | f:AAATGTTGAGCAACGGGAGCT | 59.0 | 7 | 6 | 85.71 |
r:ACTTCATAGGGCGGAGGTCT | |||||
EST-SSR-cnl144 | f:AGAAGGCGGCTCAGAAGAAG | 58.0 | 10 | 10 | 100.00 |
r:GCTCCAACTCAGAATCAACAA | |||||
EST-SSR-cnl147 | f:GGCTAGGGTTTCGACTCCTC | 60.0 | 9 | 7 | 77.78 |
r:AGATGGCGAACTCGACCTG | |||||
EST-SSR-cnl158 | f:CTCATCCCACCACCACCAC | 59.0 | 9 | 9 | 100.00 |
r:CCCTGAAGAAGTCGAACACG | |||||
Total | Â | Â | 793 | 708 | 89.28 |