Identification of novel mutations among Iranian NPC1 patients: a bioinformatics approach to predict pathogenic mutations

Table 2 primer sequences for the PCR amplification at the entire coding regions and exon/intron boundaries of the NPC1 gene

Fragment	Exon	Forward(5’-3’)	Reverse(5’-3’)	Product size(bp)	Tm (°C)
1	E1	AGCCGACGACGCCTTCTTCCTT	ACAAGTGAGGAACCTCCGAGCTC	383	61.6
2	E2	GAAGTTTCTGTGATTGTACTTGAGT	TCCACCTCCACCCTGCAATAACAT	310	61.7
3	E3	GTGTCTTAGTTCACTGAGGAATGTTG	GAAAGCTGAGCATTACCAGTTCACA	253	64.3
4	E4	TGGACACAATAAATGCAGAAAGTAAT	TGACAGGACAACTAAAAGGAACAAT	475	63.4
5	E5	AGCATGGTGCATATGGAGTTCGTG	CAAGCACTGGTGAGCCACTGTGC	369	63
6	E6	GTATTTCAGTGGGCTTTTCTTTGAGT	CATGGAGGTATTTGTTTCTTGTCCTA	475	62.5
7	E7	ACCTCACTGTGATGAAGTCCACTA	CATGACAGACAGCATCATCTGAAC	178	60.7
8	E8	TGATTCCTGCCATGAGATAGCAACT	CCCATCTAGCAGTAGTCAACATGTA	556	60.8
9	E9	ATGTGACGTGTTTCTGGGTTTGC	GTCTTGTTGTTTGCTCACCTCTG	384	62.2
10	E10	AGGTGAGTGCTGAGCTGTATTA	AGGAGATACTATTCTGGGATTCA	403	62.4
11	E11	AGATACAGTCCATAGCTCCAGTGAG	TAAGTGCTTGCTGCAAGTGTCTAGC	288	61.1
12	E12	TCGTGAAAGTTAGGGAGAAGTTT	GGCAACAGAAACGTTACATACAA	312	63.4
13	E13	TTTAGTAACAAGTGGGACAGACAAC	AGGTCACACTCACGAATGCGGAG	339	64.3
14	E14	AGTCCCCCACCGAAGTTTAT	AGCCAGCTCCTTCTTTCTCC	233	61.8
15	E15-16	GCTGTAAACAGAAGTGACGCAGA	CTGGCTTCTTAGAAGGCATGTGAT	480	62.2
16	E17	CCTGTACTCCCTATTAGCCTGTCAT	ACTTGCTTGAAACACCTACGTGCATG	322	63.2
17	E18	TGCTTAGTTACTATCAGAGTGTTCAC	CCTCCTCCGCTGCTTCTGAAGTA	291	61
18	E19	CTGTGGAGCAGGTCAGTAACCCT	GTATAAACTGAGGCACGATGCAAATG	245	62
19	E20	CTTCTAACAGTCCTCCCTGCA	CTGTCTTAGCCCAGTCCTCTC	247	64.3
20	E21	TGCTTAGCCTCAAGTGCTCAGAT	ACCCAGTGTAGGCCCTTTGCTG	337	63.7
21	E22	CATGAGAGGTCAAGTGAGTTG	ATGCTCGCTCCCTCTATG	295	62.2
22	E23	CAGGGTGCCCTGGGTAATTAGCA	GATCCAGACTCTTCAGTCACTGAG	292	61.6
23	E24	TTCAATTACAGGTTGGTAAAAGTGGTT	CTTGAAAAGAATGCCTCAGGATAGA	297	63.1
24	E25	TTCCAAAGTGGGATTACAGGCGTG	GACCGACCCTTAGACACAGTTCAG	221	64.3

ISSN: 1601-5223