Fig. 4

Expression of the CYC-B gene during fruit development in F2 generation plants. RNA was extracted from fruit samples at early (E), mature (M), turning (T), and ripe (R) developmental stages. Relative amounts of mRNA were determined by RT-qPCR after normalizing with EF1α transcript. The relative expression levels of CYC-B were compared to early stage (E) level (set to 1) in each F2 plant. Data represent an average ± s.e.m of three biological replicates and asterisk indicates values significantly different (p <0.05). a: Red, b: Orange, c: Brown, d: Orange-brown